Rapid communication: PstI, HindIII, and TaqI restriction fragment length polymorphisms in the bovine Uroplakin IB gene.

Polymorphisms. PstI, HindIII, and TuqI restriction fragment length polymorphisms (RFLP) in the bovine Uroplukin IB (UPKlB) gene. Source and Description ofProbe. The UPKlB probe is a .5-kb PCR product encoding a 27-kDa bovine Uroplukin IB protein associated with the urothelial asymmetric unit membrane (Yu et al., 1994). The insert was radiolabeled with [ c Y ~ ~ P I ~ C T P to a specific activity of > lo9 d p d p g . Hybridization Conditions. Nylon membranes were hybridized at 42°C in 5x standard sodium citrate (SSC) , 50% formamide and washed to a final stringency of . l x SSC a t 65°C. Polymorphisms. Polymorphic fragments were observed with PstI (4.5 and 4.0 kb), HindIII (3.5 and 3.0 kb), and TuqI (9.6, 6.8, and 5.3 kb; Figure 1). BunHI , BgZII, EcoRI, and MspI were invariant when tested on 18 to 25 unrelated cattle from a five-breed diallel cross. Inheritance. Autosomal codominant inheritance was confirmed in two-generation full-sib families that included 9 (PstI) , 11 (HindIII), or 10 ( TuqI) offspring. Segregation of the UPKlB alleles is illustrated (Figure 1). Allele Frequencies. Frequencies of allelic bands were determined in unrelated crossbred cattle. PstI frequencies were .46 (4.5 kb ) and .54 (4.0 kb), with an observed heterozygosity of .50 (24 animals). HindIII frequencies were .67 (3.5 kb ) and .33 (3.0 kb), with an observed heterozygosity of .44 (18 animals). TuqI frequencies were .05 (9.6 kb) , .09 (6.8 kb), and .86 (5.3 kb), with an observed heterozygosity of .25 (18 animals). Chromosomal Location. UPKlB has been mapped to bovine syntenic group U10 (bovine chromosome 1) by somatic cell hybrid analysis (Ryan et al., 1993). Probe Availability. Contact T.-T. Sun at the above address.